BD-PB4491-Phospho-CDC25A(T507) Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Phospho-CDC25A(T507) Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PB4491

产品名称

Phospho-CDC25A(T507) Rabbit Polyclonal Antibody

别名

M-phase inducer phosphatase 1, Dual specificity phosphatase Cdc25A, CDC25A

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.

Human Gene ID

NP_001780.2;NP_963861.1

Human Swissprot No.

P30304

特异性

This CDC25A Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T507 of human CDC25A.

稀释度

IF~~1:25;WB~~1:1000;IHC-P~~1:100~500

预测分子量

59kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.

功能

Tyrosine protein phosphatase which functions as a dosage- dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

期货

现货

Fluorescent image of HeLa cells stained with Phospho-CDC25A(T507) Antibody. BD-PB4491 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

Western blot analysis of extracts from Hela cells, untreated or treated with calyculin A, using Phospho-Cdc25A Antibody (T507).

The anti-Phospho-CDC25A-T507 Pab is used in Western blot to detect Phospho-CDC25A-T507 in cells transfected with wild type or mutant T507 A of CDC25A. Data courtesy of Dr. Tiebang Kang of Washington University, St. Louis, MO.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.