BD-PB4487-PRDX2 Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

PRDX2 Rabbit Polyclonal Antibody (C-term)

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PB4487

产品名称

PRDX2 Rabbit Polyclonal Antibody (C-term)

别名

Peroxiredoxin-2, Natural killer cell-enhancing factor B, NKEF-B, PRP, Thiol-specific antioxidant protein, TSA, Thioredoxin peroxidase 1, Thioredoxin-dependent peroxide reductase 1, PRDX2, NKEFB, TDPX1

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Human Gene ID

NP_005800.3

Human Swissprot No.

P32119

特异性

This PRDX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-198 amino acids from the C-terminal region of human PRDX2.

稀释度

WB~~1:1000;IF~~1:10~50;IHC-P~~1:100~500

预测分子量

22kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

PRDX2 is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. This protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. The crystal structure of this protein has been resolved to 2.7 angstroms.

细胞定位

Cytoplasm.

功能

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).

期货

现货

Western blot analysis of lysates from mouse heart and rat heart tissue lysate (from left to right), using PRDX2 Antibody (C-term) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Fluorescent confocal image of Hela cell stained with PRDX2 Antibody (C-term)was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).PRDX2 immunoreactivity is localized to Cytoplasm significantly.

Western blot analysis of PRDX2 Antibody (C-term) in MDA-MB231,293 cell line and mouse brain tissue lysates (35ug/lane).PRDX2 (arrow) was detected using the purified Pab.(2ug/ml)

Formalin-fixed and paraffin-embedded human brain tissue reacted with PRDX2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.