BD-PB4468-PTPN6 Rabbit Polyclonal Antibody (Center)-现货抗体产品库武汉枢密脑科学技术有限公司

PTPN6 Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号

BD-PB4468

产品名称

PTPN6 Rabbit Polyclonal Antibody (Center)

别名

Tyrosine-protein phosphatase non-receptor type 6, Hematopoietic cell protein-tyrosine phosphatase, Protein-tyrosine phosphatase 1C, PTP-1C, Protein-tyrosine phosphatase SHP-1, SH-PTP1, PTPN6, HCP, PTP1C

类别

常规抗体

推荐应用

反应种属

Rat, Human

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Human Gene ID

NP_002822.2;NP_536858.1;NP_536859.1

Human Swissprot No.

P29350

特异性

This PTPN6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 247-277 amino acids from the Central region of human PTPN6.

稀释度

IF~~1:100;WB~~1:1000;IHC-P~~1:100~500

预测分子量

68kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

PTPN6 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling.

组织表达

Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells

细胞定位

Cytoplasm. Nucleus. Note=In neurons, translocates into the nucleus after treatment with angiotensin II (By similarity) Shuttles between the cytoplasm and nucleus via its association with PDPK1.

功能

Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.

期货

现货

Immunofluorescent analysis of U251 cells, using PTPN6 Antibody (Center) . BD-PB4468 was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).

Western blot analysis of PTPN6 Antibody (Center) in HL-60 cell line lysates (35ug/lane).PTPN6 (arrow) was detected using the purified Pab.

Western blot analysis of PTPN6 (arrow) using rabbit polyclonal PTPN6 Antibody (Center) either nontransfected (Lane 1) or transiently transfected with the PTPN6 gene (Lane 2) .(2ug/ml)

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PTPN6 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.