BD-PB4463-Vimentin Rabbit Polyclonal Antibody (S82)-现货抗体产品库武汉枢密脑科学技术有限公司

Vimentin Rabbit Polyclonal Antibody (S82)

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产品基本信息

产品货号

BD-PB4463

产品名称

Vimentin Rabbit Polyclonal Antibody (S82)

别名

Vimentin, VIM

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.

Human Gene ID

NP_003371.2

Human Swissprot No.

P08670

特异性

This Vimentin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-90 amino acids from human Vimentin.

稀释度

WB~~1:1000;IHC-P~~1:100~500

预测分子量

54kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.

组织表达

Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

细胞定位

Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix {ECO:0000250|UniProtKB:P31000}. Cell membrane {ECO:0000250|UniProtKB:P20152}

功能

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

期货

现货

Western blot analysis of Vimentin-S82 in Hela cell line lysates (35ug/lane). Vimentin (arrow) was detected using the purified Pab.

Western blot analysis of VIM(arrow) using rabbit polyclonal Vimentin Antibody (S82) either nontransfected (Lane 1) or transiently transfected with the VIM gene (Lane 2) .

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Vimentin Antibody (S82) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.