BD-PB4462-AKR1B1 Rabbit Polyclonal Antibody (Center)-现货抗体产品库武汉枢密脑科学技术有限公司

AKR1B1 Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号

BD-PB4462

产品名称

AKR1B1 Rabbit Polyclonal Antibody (Center)

别名

Aldose reductase, AR, Aldehyde reductase, Aldo-keto reductase family 1 member B1, AKR1B1, ALDR1

类别

常规抗体

推荐应用

反应种属

Human

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Human Gene ID

NP_001619.1

Human Swissprot No.

P15121

特异性

This AKR1B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-135 amino acids from the Central region of human AKR1B1.

稀释度

WB~~1:1000;IF~~1:10~50;IHC-P~~1:100~500

预测分子量

36kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

AKR1B1 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This protein catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol.

组织表达

Highly expressed in embryonic epithelial cells (EUE) in response to osmotic stress.

细胞定位

Cytoplasm.

功能

Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosacharides, bile acids and xenobiotics substrates. Key enzyme in the polyol pathway, catalyzes reduction of glucose to sorbitol during hyperglycemia (PubMed:1936586). Reduces steroids and their derivatives and prostaglandins. Displays low enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis- retinal (PubMed:12732097, PubMed:19010934, PubMed:8343525). Catalyzes the reduction of diverse phospholipid aldehydes such as 1-palmitoyl-2- (5-oxovaleroyl)-sn -glycero-3-phosphoethanolamin (POVPC) and related phospholipid aldehydes that are generated from the oxydation of phosphotidylcholine and phosphatdyleethanolamides (PubMed:17381426). Plays a role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS- carbonyls) (PubMed:21329684).

期货

现货

All lanes : Anti-AKR1B1 Antibody (Center) at 1:8000 dilution Lane 1: A431 whole cell lysate Lane 2: A549 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of anti-AKR1B1 Pab in Jurkat cell line lysates (35ug/lane).AKR1B1 (arrow) was detected using the purified Pab.

Western blot analysis of AKR1B1 (arrow) using rabbit polyclonal AKR1B1 Antibody (Center) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the AKR1B1 gene.

Confocal immunofluorescent analysis of AKR1B1 Antibody (Center)with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with AKR1B1 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.