BD-PB4459-Aldolase (ALDOA) Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

Aldolase (ALDOA) Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB4459

产品名称

Aldolase (ALDOA) Rabbit Polyclonal Antibody (N-term)

别名

Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase, ALDOA, ALDA

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Human Gene ID

NP_000025.1;NP_001121089.1;NP_001230106.1;NP_908930.1;NP_908932.1

Human Swissprot No.

P04075

特异性

This Aldolase (ALDOA) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-95 amino acids from the N-terminal region of human Aldolase (ALDOA).

稀释度

WB~~1:1000;IHC-P~~1:100~500;IF~~1:10~50

预测分子量

39kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

Aldolase A (fructose-bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia.

细胞定位

Cytoplasm, myofibril, sarcomere, I band. Cytoplasm, myofibril, sarcomere, M line. Note=In skeletal muscle, accumulates around the M line and within the I band, colocalizing with FBP2 on both sides of the Z line in the absence of Ca(2+)

功能

Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein (By similarity).

期货

现货

Western blot analysis of lysates from 293,RD,mouse NIH/3T3,rat L6 cell line (from left to right),using ALDOA Antibody (N-term)at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ALDOA antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Confocal immunofluorescent analysis of Aldolase (ALDOA) Antibody (N-term)with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).