产品名称
UBE2D1 Rabbit Polyclonal Antibody (C-term)
别名
Ubiquitin-conjugating enzyme E2 D1, Stimulator of Fe transport, SFT, UBC4/5 homolog, UbcH5, Ubiquitin carrier protein D1, Ubiquitin-conjugating enzyme E2(17)KB 1, Ubiquitin-conjugating enzyme E2-17 kDa 1, Ubiquitin-protein ligase D1, UBE2D1, SFT, UBC5A, UBCH5, UBCH5A
存储缓冲液
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Human Gene ID
NP_003329.1
Human Swissprot No.
P51668
特异性
This UBE2D1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 amino acids from the C-terminal region of human UBE2D1.
稀释度
IHC-P~~1:100~500;WB~~1:1000
运输及保存条件
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
背景介绍
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2D1 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.
组织表达
Ubiquitous. Up-regulated in livers of iron- overloaded patients with hereditary hemochromatosis
功能
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:
22496338). In vitro catalyzes 'Lys-48'-linked polyubiquitination (PubMed:
20061386). Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6 and TRIM63/MURF1 (PubMed:
18042044, PubMed:
18359941). Ubiquitinates STUB1-associated HSP90AB1 in vitro (PubMed:
18042044). Lacks inherent specificity for any particular lysine residue of ubiquitin (PubMed:
18042044). Essential for viral activation of IRF3 (PubMed:
19854139). Mediates polyubiquitination of CYP3A4 (PubMed:
19103148).
