BD-PB4448-GDF11 Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

GDF11 Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB4448

产品名称

GDF11 Rabbit Polyclonal Antibody (N-term)

别名

Growth/differentiation factor 11, GDF-11, Bone morphogenetic protein 11, BMP-11, GDF11, BMP11

类别

常规抗体

推荐应用

反应种属

Human, Mouse

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.

Human Gene ID

NP_005802.1

Human Swissprot No.

O95390

特异性

This GDF11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-61 amino acids from the N-terminal region of human GDF11.

稀释度

WB~~1:1000;IHC-P~~1:100~500

预测分子量

45kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

GDF11 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice and Xenopus suggest that this protein is involved in mesodermal formation and neurogenesis during embryonic development.

组织表达

In the embryo, strong expression is seen in the palatal epithelia, including the medial edge epithelial and midline epithelial seam of the palatal shelves. Less pronounced expression is also seen throughout the palatal shelf and tongue mesenchyme

细胞定位

Secreted.

功能

Secreted signal that acts globally to regulate anterior/posterior axial patterning during development. May play critical roles in patterning both mesodermal and neural tissues (By similarity). It is required for proper vertebral patterning and orofacial development (PubMed:31215115). Signals through activin receptors type-2, ACVR2A and ACVR2B, and activin receptors type-1, ACVR1B, ACVR1C and TGFBR1 leading to the phosphorylation of SMAD2 and SMAD3 (PubMed:28257634).

期货

现货

All lanes : Anti-hGDF11-R47 at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: human heart lysate Lane 3: Hela whole cell lysate Lane 4: K562 whole cell lysate Lane 5: mouse brain lysate Lane 6: mouse kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of GDF11 (arrow) using rabbit polyclonal GDF11 Antibody (R47) either nontransfected (Lane 1) or transiently transfected with the GDF11 gene (Lane 2) .

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with GDF11 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.