产品名称
BAP1 Rabbit Polyclonal Antibody
基因名称
BAP1 KIAA0272 hucep-6
蛋白名称
Ubiquitin carboxyl-terminal hydrolase BAP1 (EC 3.4.19.12) (BRCA1-associated protein 1) (Cerebral protein 6)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q92560
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q92560/entry
Mouse Swissprot No.
Q99PU7
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q99PU7
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941D3ZHS6
免疫原
Synthesized peptide derived from human protein . at AA range: 150-230
特异性
BAP1 Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene belongs to the ubiquitin C-terminal hydrolase subfamily of deubiquitinating enzymes that are involved in the removal of ubiquitin from proteins. The encoded enzyme binds to the breast cancer type 1 susceptibility protein (BRCA1) via the RING finger domain of the latter and acts as a tumor suppressor. In addition, the enzyme may be involved in regulation of transcription, regulation of cell cycle and growth, response to DNA damage and chromatin dynamics. Germline mutations in this gene may be associated with tumor predisposition syndrome (TPDS), which involves increased risk of cancers including malignant mesothelioma, uveal melanoma and cutaneous melanoma. [provided by RefSeq, May 2013],
组织表达
Highly expressed in testis, placenta and ovary. Expressed in breast.
细胞定位
Cytoplasm . Nucleus . Mainly nuclear. Binds to chromatin. Localizes to the cytoplasm when monoubiquitinated by the E2/E3 hybrid ubiquitin-protein ligase UBE2O (PubMed:24703950). .
功能
catalytic activity:Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).,disease:Defects in BAP1 are found in non-small cell lung cancer (NSCLC) cell lines.,disease:Tumor-associated antigen found in several childhood medulloblastoma.,function:Deubiquitinating enzyme which may be involved in BRCA1 signal transduction pathway.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the peptidase C12 family.,subunit:Interacts with the RING finger domain of BRCA1.,tissue specificity:Highly expressed in testis, placenta and ovary. Expressed in breast.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
