产品名称
ORC2L Rabbit Polyclonal Antibody (Center)
别名
Origin recognition complex subunit 2, ORC2, ORC2L
存储缓冲液
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.
Human Gene ID
NP_006181.1
Human Swissprot No.
Q13416
特异性
This ORC2L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 208-237 amino acids from the Central region of human ORC2L.
运输及保存条件
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
背景介绍
The origin recognition complex (ORC) is a highly conserved
six subunits protein complex essential for the initiation of the
DNA replication in eukaryotic cells. Studies in yeast demonstrated
that ORC binds specifically to origins of replication and serves as
a platform for the assembly of additional initiation factors such
as Cdc6 and Mcm proteins. The protein encoded by this gene is a
subunit of the ORC complex. This protein forms a core complex with
ORC3, -4, and -5. It also interacts with CDC45 and MCM10, which are
proteins known to be important for the initiation of DNA
replication. This protein has been demonstrated to specifically
associate with the origin of replication of Epstein-Barr virus in
human cells, and is thought to be required for DNA replication from
viral origin of replication. Alternatively spliced transcript
variants have been found, one of which is a nonsense-mediated mRNA
decay candidate.
功能
Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K20me3 and H4K27me3. Stabilizes LRWD1, by protecting it from ubiquitin-mediated proteasomal degradation. Also stabilizes ORC3.
