BD-PB4362-ALDH2 Rabbit Polyclonal Antibody (Center)-现货抗体产品库武汉枢密脑科学技术有限公司

ALDH2 Rabbit Polyclonal Antibody (Center)

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PB4362

产品名称

ALDH2 Rabbit Polyclonal Antibody (Center)

别名

Aldehyde dehydrogenase, mitochondrial, ALDH class 2, ALDH-E2, ALDHI, ALDH2, ALDM

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.

Human Gene ID

NP_000681.2;NP_001191818.1

Human Swissprot No.

P05091

特异性

This ALDH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 318-347 amino acids from the Central region of human ALDH2.

稀释度

WB~~1:1000;IHC-P~~1:100~500

预测分子量

56kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

ALDH2 belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Asians have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Asians than among Caucasians could be related to the absence of the mitochondrial isozyme.

细胞定位

Mitochondrion matrix.

期货

现货

All lanes : Anti-ALDH2 Antibody (Center) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: A549 whole cell lysate Lane 3: Mouse liver tissue lysate Lane 4: Mouse lung tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 56 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

ALDH2 Antibody (Center) western blot analysis in A549 cell line,mouse liver and lung,rat liver lysates (35ug/lane).This demonstrates the ALDH2 antibody detected the ALDH2 protein (arrow).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ALDH2 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.