BD-PB4343-MCL1 Rabbit Polyclonal Antibody (BH3 Domain Specific)-现货抗体产品库武汉枢密脑科学技术有限公司

MCL1 Rabbit Polyclonal Antibody (BH3 Domain Specific)

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产品基本信息

产品货号

BD-PB4343

产品名称

MCL1 Rabbit Polyclonal Antibody (BH3 Domain Specific)

别名

Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3, Bcl2-L-3, Bcl-2-related protein EAT/mcl1, mcl1/EAT, MCL1, BCL2L3

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.

Human Gene ID

NP_001184249.1;NP_068779.1;NP_877495.1

Human Swissprot No.

Q07820

特异性

This MCL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 191-226 amino acids from human MCL1.

稀释度

FC~~1:25;WB~~1:1000;IHC-P~~1:100~500

预测分子量

37kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

The Mcl-1 protein belongs to the Bcl-2 family. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing.

细胞定位

Membrane; Single-pass membrane protein. Cytoplasm. Mitochondrion. Nucleus, nucleoplasm Note=Cytoplasmic, associated with mitochondria

功能

Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.

期货

现货

Overlay histogram showing A431 cells stained with BD-PB4343 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

All lanes : Anti-MCL1 Antibody (BH3 Domain Specific) at 1:2000 dilution Lane 1: Raji whole cell lysate Lane 2: Ramos whole cell lysate Lane 3: A431 whole cell lysate Lane 4: F9 whole cell lysate Lane 5: rat heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of anti-Mcl-1 BH3 Domain Pab in Ramos cell line lysates (35ug/lane). Mcl-1-BH3(arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human breast carcinoma with MCL1 Antibody (BH3 Domain Specific), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.