BD-PB4341-Bak Rabbit Polyclonal Antibody (BH3 Domain Specific)-现货抗体产品库武汉枢密脑科学技术有限公司

Bak Rabbit Polyclonal Antibody (BH3 Domain Specific)

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产品基本信息

产品货号

BD-PB4341

产品名称

Bak Rabbit Polyclonal Antibody (BH3 Domain Specific)

别名

Bcl-2 homologous antagonist/killer, Apoptosis regulator BAK, Bcl-2-like protein 7, Bcl2-L-7, BAK1, BAK, BCL2L7, CDN1

类别

常规抗体

推荐应用

反应种属

Human

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.

Human Gene ID

NP_001179.1

Human Swissprot No.

Q16611

特异性

This Bak antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-91 amino acids from human Bak.

稀释度

WB~~1:2000;IHC-P~~1:100~500

预测分子量

23kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

BAK belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BAK localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.

组织表达

Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle

细胞定位

Mitochondrion outer membrane; Single-pass membrane protein

功能

Plays a role in the mitochondrial apoptosic process. Upon arrival of cell death signals, promotes mitochondrial outer membrane (MOM) permeabilization by oligomerizing to form pores within the MOM. This releases apoptogenic factors into the cytosol, including cytochrome c, promoting the activation of caspase 9 which in turn processes and activates the effector caspases.

期货

现货

All lanes : Anti-hBak-BH3 at 1:2000 dilution Lane 1: THP-1 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: MDA-MB-453 whole cell lysate Lane 4: 293 whole cell lysate Lane 5: RPMI 8226 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.