产品名称
ZP4 Rabbit Polyclonal Antibody (C-term)
别名
Zona pellucida sperm-binding protein 4, Zona pellucida glycoprotein 4, Zp-4, Zona pellucida protein B, Processed zona pellucida sperm-binding protein 4, ZP4, ZPB
存储缓冲液
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.
Human Gene ID
NP_067009.1
Human Swissprot No.
Q12836
特异性
This ZP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-474 amino acids from the C-terminal region of human ZP4.
稀释度
WB~~1:1000;IHC-P~~1:100~500
运输及保存条件
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
背景介绍
The zona pellucida is an extracellular matrix that
surrounds the oocyte and early embryo. It is composed primarily of
three or four glycoproteins with various functions during
fertilization and preimplantation development. The nascent protein
contains a N-terminal signal peptide sequence, a conserved ZP
domain, a consensus furin cleavage site, and a C-terminal
transmembrane domain. It is hypothesized that furin cleavage
results in release of the mature protein from the plasma membrane
for subsequent incorporation into the zona pellucida matrix.
However, the requirement for furin cleavage in this process remains
controversial based on mouse studies. Previously, this gene has
been referred to as ZP1 or ZPB and thought to have similar
functions as mouse Zp1. However, a human gene with higher
similarity and chromosomal synteny to mouse Zp1 has been assigned
the symbol ZP1 and this gene has been assigned the symbol ZP4.
组织表达
Expressed in oocytes.
细胞定位
[Processed zona pellucida sperm-binding protein 4]: Zona pellucida {ECO:0000250|UniProtKB:Q00193}
功能
Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP4 may act as a sperm receptor.
