BD-PB4301-DBH Rabbit Polyclonal Antibody (N-term P42)-现货抗体产品库武汉枢密脑科学技术有限公司

DBH Rabbit Polyclonal Antibody (N-term P42)

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产品基本信息

产品货号

BD-PB4301

产品名称

DBH Rabbit Polyclonal Antibody (N-term P42)

别名

Dopamine beta-hydroxylase, Dopamine beta-monooxygenase, Soluble dopamine beta-hydroxylase, DBH

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.

Human Gene ID

NP_000778.3

Human Swissprot No.

P09172

特异性

This DBH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-56 amino acids from the N-terminal region of human DBH.

稀释度

IF~~1:25;WB~~1:2000

预测分子量

69kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. [provided by RefSeq].

细胞定位

[Soluble dopamine beta-hydroxylase]: Cytoplasmic vesicle, secretory vesicle lumen Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen. Secreted

功能

Conversion of dopamine to noradrenaline.

期货

现货

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling DBH with BD-PB4301 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and weak nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).

All lanes : Anti-DBH Antibody (N-term P42) at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: mouse adrenal gland lysate Lane 3: rat adrenal gland lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 69 kDa Blocking/Dilution buffer: 5% NFDM/TBST.