BD-PB4290-ETS1 Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

ETS1 Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB4290

产品名称

ETS1 Rabbit Polyclonal Antibody (N-term)

别名

Protein C-ets-1, p54, ETS1, EWSR2

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.

Human Gene ID

NP_001137292.1;NP_001155894.1;NP_005229.1

Human Swissprot No.

P14921

特异性

This ETS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-70 amino acids from the N-terminal region of human ETS1.

稀释度

WB~~1:1000;FC~~1:10~50;IF~~1:10~50

预测分子量

50kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

ETS transcriptions factors, such as ETS1, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes (summary by Dwyer et al., 2007 [PubMed 17986575]).

组织表达

Highly expressed within lymphoid cells. Isoforms c- ETS-1A and Ets-1 p27 are both detected in all fetal tissues tested, but vary with tissue type in adult tissues. None is detected in brain or kidney.

细胞定位

Cytoplasm. Nucleus Note=Delocalizes from nucleus to cytoplasm when coexpressed with isoform Ets-1 p27.

功能

Transcription factor. Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts. May control the differentiation, survival and proliferation of lymphoid cells. May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion.

期货

现货

All lanes : Anti-ETS1 Antibody (N-term) at 1:1000 dilution Lane 1: Jurkat whole cell lysates Lane 2: 293T whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

ETS1 Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of ETS1 Antibody (N-term)with MCF-7 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).