产品名称
Dnmt3a Rabbit Polyclonal Antibody
别名
DNA (cytosine-5)-methyltransferase 3A, Dnmt3a, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, MHsaIIIA, DNMT3A
存储缓冲液
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.
Human Gene ID
NP_072046.2;NP_715640.2;NP_783328.1;NP_783329.1
Human Swissprot No.
Q9Y6K1
特异性
This Dnmt3a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 457-486 amino acids from human Dnmt3a.
运输及保存条件
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
背景介绍
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3a is a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.
组织表达
Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung
细胞定位
Nucleus. Chromosome Cytoplasm. Note=Accumulates in the major satellite repeats at pericentric heterochromatin {ECO:0000250|UniProtKB:O88508}
功能
Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development (PubMed:
12138111, PubMed:
16357870, PubMed:
30478443). DNA methylation is coordinated with methylation of histones (PubMed:
12138111, PubMed:
16357870, PubMed:
30478443). It modifies DNA in a non-processive manner and also methylates non-CpG sites (PubMed:
12138111, PubMed:
16357870, PubMed:
30478443). May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1 (By similarity). Plays a role in paternal and maternal imprinting (By similarity). Required for methylation of most imprinted loci in germ cells (By similarity). Acts as a transcriptional corepressor for ZBTB18 (By similarity). Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites (By similarity). Can actively repress transcription through the recruitment of HDAC activity (By similarity). Also has weak auto-methylation activity on Cys-710 in absence of DNA (By similarity).
