BD-PB4269-ENOG (5W16) Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

ENOG (5W16) Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PB4269

产品名称

ENOG (5W16) Mouse Monoclonal Antibody

别名

Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2

类别

常规抗体

推荐应用

反应种属

Human

存储缓冲液

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein G column, followed by dialysis against PBS.

Human Gene ID

NP_001966.1

Human Swissprot No.

P09104

特异性

This ENOG monoclonal antibody is generated from mouse immunized with ENOG recombinant protein.

稀释度

WB~~1:1000;IHC~~1:2000;IHC-P~~1:100~500

预测分子量

47kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Mouse

同种型

IgG2b,k

背景介绍

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.

组织表达

The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons

细胞定位

Cytoplasm. Cell membrane. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form

功能

Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium- dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).

期货

现货

All lanes : Anti-ENOG at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: CEM whole cell lysate Lane 3: U-251 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded Human pancreas section using Pink1. BD-PB4269 was diluted at 1:2000 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

ENOG Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ENOG Antibody for immunohistochemistry. Clinical relevance has not been evaluated.