BD-PB4266-ALDH2 (9B9) Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

ALDH2 (9B9) Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PB4266

产品名称

ALDH2 (9B9) Mouse Monoclonal Antibody

别名

Aldehyde dehydrogenase, mitochondrial, ALDH class 2, ALDH-E2, ALDHI, ALDH2, ALDM

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

存储缓冲液

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein G column, followed by dialysis against PBS.

Human Gene ID

NP_000681.2;NP_001191818.1

Human Swissprot No.

P05091

特异性

This ALDH2 Monoclonal antibody is generated from mouse immunized with ALDH2 recombinant protein.

稀释度

WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50;IF~~1:10~50

预测分子量

56kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Mouse

同种型

IgG1,Igκ

背景介绍

This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix.

细胞定位

Mitochondrion matrix.

期货

现货

Western blot analysis of lysates from NCI-H292, A549, HepG2 cell line (from left to right), using ALDH2 Antibodyat 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.

ALDH2 Monoclonal Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ALDH2 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

ALDH2 Monoclonal Antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of ALDH2 Antibody with HepG2 cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).