BD-PB4265-BRAF (8O19) Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

BRAF (8O19) Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PB4265

产品名称

BRAF (8O19) Mouse Monoclonal Antibody

别名

Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, BRAF, BRAF1, RAFB1

类别

常规抗体

推荐应用

反应种属

Human

存储缓冲液

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein G column, followed by dialysis against PBS.

Human Gene ID

NP_004324.2

Human Swissprot No.

P15056

特异性

This BRAF Monoclonal antibody is generated from mouse immunized with BRAF recombinant protein.

稀释度

WB~~1:500~2000;IF~~1:10~50

预测分子量

84kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Mouse

同种型

IgG1κ

背景介绍

This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.

组织表达

Brain and testis.

细胞定位

Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.

功能

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126). May play a role in the postsynaptic responses of hippocampal neurons (PubMed:1508179).

期货

现货

All lanes : Anti-BRAF Antibody at 1:1000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent image of C2C12 cell stained with BRAF Antibodywas used (1:400, 50 min at 37℃.Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃.BRAF immunoreactivity is localized to Cytoplasm significantly.

Western blot analysis of anti-BRAF Antibody in HL-60 cell line lysates (35μg/lane). BRAF (arrow) was detected using the purified Mab.