BD-PB4203-CD276 (19L8) Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CD276 (19L8) Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PB4203

产品名称

CD276 (19L8) Mouse Monoclonal Antibody

别名

CD276 antigen, 4Ig-B7-H3, B7 homolog 3, B7-H3, Costimulatory molecule, CD276, CD276, B7H3

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Swissprot No.

Q5ZPR3

特异性

This CD276 antibody is generated from a mouse immunized with a recombinant protein from the human region of human CD276.

稀释度

IHC-P~~1:500;IHC~~1:400;WB~~1:4000

预测分子量

57kDa

同种型

IgG1,κ

背景介绍

May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.

组织表达

Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta.

细胞定位

Membrane; Single-pass type I membrane protein

期货

现货

Immunohistochemical analysis of CD276 Antibody in MCF-7 cell sections. Cell was fixed with formaldehyde and blocked with super block for 10 minutes at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:25) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded Human tonsil section using CD276 Antibody. BD-PB4203 was diluted at 1:400 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of CD276 Antibody in 293T(untransfected) cell sections. Cell was fixed with formaldehyde and blocked with super block for 10 minutes at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

All lanes : Anti-CD276 Antibody at 1:4000 dilution Lane 1: 293T-17 whole cell lysate Lane 2: A-431 whole cell lysate Lane 3: U-20S whole cell lysate Lane 4: LNCap whole cell lysate Lane 5: Hela whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.