BD-PB4175-CAMK2 beta (4U17) Mouse Monoclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

CAMK2 beta (4U17) Mouse Monoclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB4175

产品名称

CAMK2 beta (4U17) Mouse Monoclonal Antibody (C-term)

别名

Calcium/calmodulin-dependent protein kinase type II subunit beta, CaM kinase II subunit beta, CaMK-II subunit beta, 2.7.11.17, CAMK2B, CAM2, CAMK2, CAMKB

类别

常规抗体

推荐应用

反应种属

Human, Mouse, Rat

Human Swissprot No.

Q13554

特异性

This CAMK2 beta antibody is generated from a mouse immunized with a recombinant protein between 1-503 amino acids from human CAMK2 beta.

稀释度

IHC-P~~1:100~500;WB~~1:2000

预测分子量

73kDa

同种型

IgG1

背景介绍

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca(2+) transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2.

组织表达

Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Expressed in skeletal muscle.

细胞定位

Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse {ECO:0000250|UniProtKB:P08413}. Note=In slow-twitch muscle, evenly distributed between longitudinal SR and junctional SR

期货

现货

BD-PB4175 staining CAMK2 in human cerebellum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

All lanes : Anti-CAMK2 beta Antibody (C-term) at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: PC-12 whole cell lysate Lane 3: human brain lysate Lane 4: mouse heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 60 kDa Blocking/Dilution buffer: 5% NFDM/TBST.