BD-PB4144-RAB1B (12A19) Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

RAB1B (12A19) Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PB4144

产品名称

RAB1B (12A19) Mouse Monoclonal Antibody

别名

Ras-related protein Rab-1B, RAB1B

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Swissprot No.

Q9H0U4

特异性

This RAB1B antibody is generated from a mouse immunized with a recombinant protein between 1-201 amino acids from human RAB1B.

稀释度

FC~~1:25;WB~~1:4000

预测分子量

22kDa

同种型

IgG1,k

背景介绍

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB1B regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. Plays a role in the initial events of the autophagic vacuole development which take place at specialized regions of the endoplasmic reticulum.

细胞定位

Cytoplasm. Membrane; Lipid-anchor; Cytoplasmic side. Preautophagosomal structure membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, perinuclear region {ECO:0000250|UniProtKB:P10536}. Note=Targeted by REP1 to membranes of specific subcellular compartments including endoplasmic reticulum, Golgi apparatus, and intermediate vesicles between these two compartments (PubMed:11389151). In the GDP-form, colocalizes with GDI in the cytoplasm (PubMed:11389151). Co-localizes with MTMR6 to the endoplasmic reticulum-Golgi intermediate compartment and to the peri- Golgi region (By similarity). {ECO:0000250|UniProtKB:P10536, ECO:0000269|PubMed:11389151}

期货

现货

Overlay histogram showing A431 cells stained with BD-PB4144(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

All lanes : Anti-RAB1B Antibody at 1:4000 dilution Lane 1: Hela whole cell lysate Lane 2: A431 whole cell lysate Lane 3: U-20S whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.