BD-PB4130-MITF (12A4) Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

MITF (12A4) Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PB4130

产品名称

MITF (12A4) Mouse Monoclonal Antibody

别名

Microphthalmia-associated transcription factor, Class E basic helix-loop-helix protein 32, bHLHe32, MITF, BHLHE32

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Swissprot No.

O75030

特异性

This MITF antibody is generated from a mouse immunized with a recombinant protein of human MITF.

稀释度

WB~~1:2000;IHC~~1:1000

预测分子量

59kDa

同种型

IgG1,k

背景介绍

Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5'- CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest- derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

组织表达

Expressed in melanocytes (at protein level). [Isoform C2]: Expressed in the kidney and retinal pigment epithelium. [Isoform H2]: Expressed in the kidney. [Isoform Mdel]: Expressed in melanocytes.

细胞定位

Nucleus. Cytoplasm Note=Found exclusively in the nucleus upon phosphorylation

期货

现货

All lanes : Anti-MITF Antibody at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: PC-12 whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 59 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded Human Melanoma section using Pink1. BD-PB4130 was diluted at 1:1000 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.