BD-PB4129-MCM3 (15G18) Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

MCM3 (15G18) Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PB4129

产品名称

MCM3 (15G18) Mouse Monoclonal Antibody

别名

DNA replication licensing factor MCM3, DNA polymerase alpha holoenzyme-associated protein P1, P1-MCM3, RLF subunit beta, p102, MCM3

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Swissprot No.

P25205

特异性

This MCM3 antibody is generated from a mouse immunized with a recombinant protein of human MCM3.

稀释度

FC~~1:25;WB~~1:4000

预测分子量

91kDa

同种型

IgG1,k

背景介绍

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.

细胞定位

Nucleus {ECO:0000250|UniProtKB:P49739}. Chromosome {ECO:0000250|UniProtKB:P49739}. Note=Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses. {ECO:0000250|UniProtKB:P49739}

期货

现货

Overlay histogram showing Hela cells stained with BD-PB4129(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

All lanes : Anti-MCM3 Antibody at 1:4000 dilution Lane 1: CHO whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: C2C12 whole cell lysate Lane 6: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 91 kDa Blocking/Dilution buffer: 5% NFDM/TBST.