BD-PB4082-SP1 (5H2) Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

SP1 (5H2) Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PB4082

产品名称

SP1 (5H2) Mouse Monoclonal Antibody

别名

Transcription factor Sp1, SP1, TSFP1

类别

常规抗体

推荐应用

反应种属

Human

Human Swissprot No.

P08047

特异性

This SP1 antibody is generated from a mouse immunized with a recombination protein from the human SP1.

稀释度

FC~~1:25;IF~~1:25;WB~~1:1000

预测分子量

81kDa

同种型

IgG1,κ

背景介绍

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR- alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression.

组织表达

Up-regulated in adenocarcinomas of the stomach (at protein level). Isoform 3 is ubiquitously expressed at low levels

细胞定位

Nucleus. Cytoplasm. Note=Nuclear location is governed by glycosylated/phosphorylated states. Insulin promotes nuclear location, while glucagon favors cytoplasmic location

期货

现货

Flow cytometric analysis of Hela cells using SP1 Antibody(green) compared to an isotype control of mouse IgG1(blue). BD-PB4082 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.

Fluorescent image of Hela cells stained with SP1 Antibody. BD-PB4082 was diluted at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

Western blot analysis of lysates from Hela, Ramos cell line (from left to right), using SP1 Antibodyat 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.