BD-PB4052-MGMT (12H16) Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

MGMT (12H16) Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PB4052

产品名称

MGMT (12H16) Mouse Monoclonal Antibody

别名

Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, MGMT, O-6-methylguanine-DNA-alkyltransferase, MGMT

类别

常规抗体

推荐应用

反应种属

Human

Human Swissprot No.

P16455

特异性

Purified His-tagged MGMT protein was used to produced this monoclonal antibody.

稀释度

IHC-P~~1:100~500;IF~~1:25;WB~~1:1000;IHC~~1:100

预测分子量

22kDa

同种型

IgG1

背景介绍

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

细胞定位

Nucleus.

期货

现货

Immunohistochemical analysis of paraffin-embedded H. small intestine section using MGMT Antibody. BD-PB4052 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Fluorescent image of Hela cells stained with MGMT Antibody. BD-PB4052 was diluted at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

MGMT Antibodywestern blot analysis in MCF-7 cell line lysates (35μg/lane).This demonstrates the MGMT antibody detected the MGMT protein (arrow).

Immunohistochemical analysis of paraffin-embedded Human Prostate cancer section using Pink1. BD-PB4052 was diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.