BD-PB4019-Cyclin A (CCNA2) Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

Cyclin A (CCNA2) Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB4019

产品名称

Cyclin A (CCNA2) Rabbit Polyclonal Antibody (N-term)

别名

Cyclin-A2, Cyclin-A, CCNA2, CCN1, CCNA

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_001228.1

Human Swissprot No.

P20248

特异性

This Cyclin A (CCNA2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-84 amino acids from the N-terminal region of human Cyclin A (CCNA2).

稀释度

WB~~1:2000;IHC-P~~1:100~500;FC~~1:10~50

预测分子量

49kDa

同种型

Rabbit IgG

背景介绍

CCNA2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.

细胞定位

Nucleus. Cytoplasm. Note=Exclusively nuclear during interphase (PubMed:1312467). Detected in the nucleus and the cytoplasm at prophase (PubMed:1312467). Cytoplasmic when associated with SCAPER (PubMed:17698606).

期货

现货

All lanes : Anti-CCNA2 Antibody (N-term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: K562 whole cell lysate Lane 3: MGC803 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 49 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human hepatocarcinoma with Cyclin A (CCNA2) Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of HepG2 cells using Cyclin A (CCNA2) Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.