BD-PB4010-RNGTT Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

RNGTT Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB4010

产品名称

RNGTT Rabbit Polyclonal Antibody (C-term)

别名

mRNA-capping enzyme, HCAP1, HCE, Polynucleotide 5'-triphosphatase, mRNA 5'-triphosphatase, TPase, mRNA guanylyltransferase, GTP--RNA guanylyltransferase, GTase, RNGTT, CAP1A

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Gene ID

NP_003791.3

Human Swissprot No.

O60942

特异性

This RNGTT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 489-516 amino acids from the C-terminal region of human RNGTT.

稀释度

WB~~1:1000;FC~~1:10~50

预测分子量

69kDa

同种型

Rabbit IgG

背景介绍

RNGTT is a bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. The protein catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus.

组织表达

Isoform 1 and isoform 4 (at a lesser extent) are expressed in cerebrum, cerebellum, thyroid, lung, heart, liver, kidney, spleen, large intestine, testis, skin and muscle

细胞定位

Nucleus.

期货

现货

All lanes : Anti-RNGTT Antibody (C-term) at 1:1000 dilution Lane 1: HL-60 whole cell lysate Lane 2: rat testis tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution. Observed band size : 69kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of RNGTT antibody (C-term) in 293 cell line lysates (35ug/lane). RNGTT (arrow) was detected using the purified Pab.

Flow cytometric analysis of 293 cells using RNGTT Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.