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ALB Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号
BD-PB4006
产品名称
ALB Rabbit Polyclonal Antibody (C-term)
别名
Serum albumin, ALB
类别
常规抗体
推荐应用
WB
反应种属
Human, Rat, Mouse
Human Gene ID
NP_000468.1
Human Swissprot No.
P02768
特异性
This ALB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 540-569 amino acids from the C-terminal region of human ALB.
稀释度
IHC-P-Leica~~1:500;WB~~1:1000
预测分子量
69kDa
同种型
Rabbit IgG
背景介绍
Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
组织表达
Plasma.
细胞定位
Secreted.
期货
现货

Immunohistochemical analysis of paraffin-embedded Human kidney tissue using BD-PB4006 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded Human liver tissue using BD-PB4006 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes : Anti-ALB Antibody (C-term) at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Mouse liver tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 69 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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