BD-PB3993-PHB Rabbit Polyclonal Antibody (C-term E194)-现货抗体产品库武汉枢密脑科学技术有限公司

PHB Rabbit Polyclonal Antibody (C-term E194)

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产品基本信息

产品货号

BD-PB3993

产品名称

PHB Rabbit Polyclonal Antibody (C-term E194)

别名

Prohibitin, PHB

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_001268425.1;NP_001268644.1;NP_002625.1

Human Swissprot No.

P35232

特异性

This PHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 179-205 amino acids from the C-terminal region of human PHB.

稀释度

WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50

预测分子量

30kDa

同种型

Rabbit IgG

背景介绍

Prohibitin is an evolutionarily conserved protein that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region.

组织表达

Widely expressed in different tissues.

细胞定位

Mitochondrion inner membrane. Nucleus. Cytoplasm. Cell membrane

期货

现货

Western blot analysis of PHB (arrow) using rabbit polyclonal PHB Antibody (C-term) either nontransfected (Lane 1) or transiently transfected with the PHB gene (Lane 2) .

Western blot analysis of anti-PHB Antibody (C-term) in Jurkat cell line lysates (35ug/lane).PHB (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PHB Antibody (C-term E194), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of NCI-H292 cells using PHB Antibody (C-term E194)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.