产品名称
Mouse Chek1 Rabbit Polyclonal Antibody (Center)
别名
Serine/threonine-protein kinase Chk1, CHK1 checkpoint homolog, Checkpoint kinase-1, Chek1, Chk1
Human Swissprot No.
O35280
Mouse Gene ID
NP_031717.2
特异性
This Mouse Chek1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 252-279 amino acids from the Central region of mouse Chek1.
背景介绍
Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-171' and 'Thr-497' and phosphorylation of CDC25C creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-74', 'Ser-122', 'Ser-171', 'Ser-271' and 'Ser-284' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation (By similarity). Essential for early embryogenesis.
组织表达
Found in all adult tissues tested. Elevated expression in testis, lung and spleen. 15.5 day old embryos show ubiquitous expression with strong expression in brain, liver, kidney, pancreas, intestine, thymus and lung.
细胞定位
Nucleus. Chromosome. Cytoplasm {ECO:0000250|UniProtKB:O14757} Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250|UniProtKB:O14757}. Note=Nuclear export is mediated at least in part by XPO1/CRM1. Also localizes to the centrosome specifically during interphase, where it may protect centrosomal CDC2 kinase from inappropriate activation by cytoplasmic CDC25B. Proteolytic cleavage at the C-terminus by SPRTN promotes removal from chromatin {ECO:0000250|UniProtKB:O14757}
