BD-PB3944-TRPV1 Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

TRPV1 Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB3944

产品名称

TRPV1 Rabbit Polyclonal Antibody (N-term)

别名

Transient receptor potential cation channel subfamily V member 1, TrpV1, Capsaicin receptor, Osm-9-like TRP channel 1, OTRPC1, Vanilloid receptor 1, TRPV1, VR1

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Gene ID

NP_061197.4;NP_542435.2;NP_542436.2;NP_542437.2

Human Swissprot No.

Q8NER1

特异性

This TRPV1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124-153 amino acids from the N-terminal region of human TRPV1.

稀释度

IHC-P-Leica~~1:500;WB~~1:2000

预测分子量

95kDa

同种型

Rabbit IgG

背景介绍

Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene.

组织表达

Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level)

细胞定位

Cell junction, synapse, postsynaptic cell membrane {ECO:0000250|UniProtKB:O35433}; Multi-pass membrane protein {ECO:0000250|UniProtKB:O35433}. Cell projection, dendritic spine membrane {ECO:0000250|UniProtKB:O35433}; Multi-pass membrane protein {ECO:0000250|UniProtKB:O35433}. Cell membrane; Multi-pass membrane protein {ECO:0000250|UniProtKB:O35433}. Note=Mostly, but not exclusively expressed in postsynaptic dendritic spines {ECO:0000250|UniProtKB:O35433}

期货

现货

Immunohistochemical analysis of paraffin-embedded human kidney tissue using BD-PB3944 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded human brain tissue using BD-PB3944 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes : Anti-TRPV1 Antibody (N-term) at 1:2000 dilution Lane 1: Rat cerebellum lysate Lane 2: Mouse cerebellum lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 95 kDa Blocking/Dilution buffer: 5% NFDM/TBST.