BD-PB3933-HtrA3 Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

HtrA3 Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB3933

产品名称

HtrA3 Rabbit Polyclonal Antibody (C-term)

别名

Serine protease HTRA3, 3421-, High-temperature requirement factor A3, Pregnancy-related serine protease, HTRA3, PRSP

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Gene ID

NP_444272.1

Human Swissprot No.

P83110

特异性

This HtrA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 394-425 amino acids from the C-terminal region of human HtrA3.

稀释度

WB~~1:1000;IHC-P~~1:100~500

预测分子量

49kDa

同种型

Rabbit IgG

背景介绍

Insulin-like growth factors (IGFs) stimulate the proliferation and differentiation of a vast number of cell types. The action of the growth factors is mediated and controlled by a complex system of components, including several proteases that cleave the IGF-Binding Proteins. HtrA1 is a 480 aa protein that contains an N-terminus homologous to MAC25 (IGFBP7) with a conserved Kazal-type serine protease inhibitor motif, as well as a C-terminal PDZ domain. Semiquantitative RT-PCR and immunoblot analyses showed an approximately 7-fold increase of PRSS11 in osteoarthritis cartilage compared with controls. HTRA2 is released from mitochondria and inhibits the function of XIAP by direct binding in a way similar to SMAC. Moreover, when overexpressed extramitochondrially, HTRA2 induced atypical cell death, which was neither accompanied by a significant increase in caspase activity nor inhibited by caspase inhibitors, including XIAP. A catalytically inactive mutant of HTRA2, however, did not induce cell death. Suzuki et al. (2001) concluded that HTRA2 is a SMAC-like inhibitor of IAP (inhibitor of apoptosis proteins) activity with a serine protease-dependent cell death-inducing activity.

组织表达

Widely expressed, with highest levels in both adult and fetal heart, ovary, uterus placenta, and bladder. In the endometrium, expressed in epithelial glands and the stroma. Also present in leukocytes. Isoform 1 is predominant in heart and skeletal muscle, whereas isoform 2 is predominant in placenta and kidney

细胞定位

Secreted. Note=Secretion increased during decidualization of endometrial stromal cells

期货

现货

All lanes : Anti-HtrA3 Antibody (C-term) at 1:1000 dilution Lane 1:mouse brain whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Observed band size : 51kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of anti-HtrA3 C-term Pab in mouse brain tissue lysate. HtrA3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.