Western blot analysis of lysate from SOX2 protein, using SOX2 Antibodyat 1:3000 dilution was used as the secondary antibody. Lysate at 20μg.

Fluorescent image of A549 cell stained with SOX2 Antibodywas used (1:400, 50 min at 37℃.Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃.SOX2 immunoreactivity is localized to Nucleus significantly.

Western blot analysis of SOX2 Antibody by SOX2 recombinant protein. SOX2(arrow) was detected using the purified Mab.

Western blot analysis of SOX2 (arrow) using mouse monoclonal SOX2 antibody either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2)

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of NCI-H460 cells using SOX2 Monoclonal Antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.

Immunohistochemical analysis of paraffin-embedded Human Lung squamous cell carcinoma section using Pink1. BD-PB3860 was diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.