BD-PB3825-BMI1 (18X10) Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

BMI1 (18X10) Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PB3825

产品名称

BMI1 (18X10) Mouse Monoclonal Antibody

别名

Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RING finger protein 51, BMI1, PCGF4, RNF51

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_001190991.1;NP_005171.4

Human Swissprot No.

P35226

特异性

This BMI1 monoclonal antibody is generated from mouse immunized with BMI1 recombinant protein.

稀释度

WB~~1:500~1000;IF~~1:10~50

预测分子量

37kDa

同种型

IgG1,k

背景介绍

Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

细胞定位

Nucleus. Cytoplasm

期货

现货

All lanes : Anti-BMI1 Antibody at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: A549 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

BMI1 Antibody western blot analysis in K562 cell line lysates (35μg/lane).This demonstrates the BMI1 antibody detected the BMI1 protein (arrow).

Confocal immunofluorescent analysis of BMI1 Antibody with NCI-H460 cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green).Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).