BD-PB3800-RET (18T17) Mouse Monoclonal Antibody (Ascites)-现货抗体产品库武汉枢密脑科学技术有限公司

RET (18T17) Mouse Monoclonal Antibody (Ascites)

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产品基本信息

产品货号

BD-PB3800

产品名称

RET (18T17) Mouse Monoclonal Antibody (Ascites)

别名

Proto-oncogene tyrosine-protein kinase receptor Ret, Cadherin family member 12, Proto-oncogene c-Ret, Soluble RET kinase fragment, Extracellular cell-membrane anchored RET cadherin 120 kDa fragment, RET, CDHF12, CDHR16, PTC, RET51

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_065681.1;NP_066124.1

Human Swissprot No.

P07949

特异性

This RET Monoclonal antibody was raised using purified His-tagged recombinant human RET.

稀释度

WB~~ 1:1000;IHC-P~~1:100~500;IF~~1:10~50

预测分子量

124kDa

同种型

IgM,K

背景介绍

This gene, a member of the cadherin superfamily, encodes one of the receptor tyrosine kinases, which are cell-surface molecules that transduce signals for cell growth and differentiation. This gene plays a crucial role in neural crest development, and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in this gene are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their biological validity has not been confirmed.

细胞定位

Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein Note=Predominantly located on the plasma membrane. In the presence of SORL1 and GFRA1, directed to endosomes.

期货

现货

RET western blot analysis in A549 cell line lysates (35μg/lane).This demonstrates the RET antibody detected the RET protein (arrow).

RET Monoclonal( Ascites) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RET Monoclonal( Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.

Confocal immunofluorescent analysis of RET Antibody (Ascites)with MDA-MB231 cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green).Actin filaments have been labeled with Alexa Fluor? 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Anti-RET Antibody (Ascites) at 1:1000 dilution + mouse heart lysate Secondary Goat Anti-mouse IgM, (H+L),Peroxidase conjugated at 1/10000 dilution. Predicted band size : 124319 Da Blocking/Dilution buffer: 5% NFDM/TBST.