产品名称
PHF8 Rabbit Polyclonal Antibody
别名
Histone lysine demethylase PHF8 (EC 1.14.11.27) (PHD finger protein 8)
基因名称
PHF8 KIAA1111 ZNF422
蛋白名称
Histone lysine demethylase PHF8 (EC 1.14.11.27) (PHD finger protein 8)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=23133
Human Swissprot No.
Q9UPP1
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9UPP1/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=320595
Mouse Swissprot No.
Q80TJ7
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q80TJ7/entry
特异性
This antibody detects endogenous levels of PHF8 at Human, Mouse
稀释度
WB 1:500-2000 IHC 1:50-200
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
细胞定位
Nucleus . Nucleus, nucleolus . Recruited to H3K4me3 sites on chromatin during interphase (PubMed:20622854). Dissociates from chromatin when cells enter mitosis (PubMed:20622854). .
功能
Histone lysine demethylase with selectivity for the di- and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Acts as a coactivator of rDNA transcription, by activating polymerase I (pol I) mediated transcription of rRNA genes. Required for brain development, probably by regulating expression of neuron-specific genes. Only has activity toward H4K20Me1 when nucleosome is used as a substrate and when not histone octamer is used as substrate. May also have weak activity toward dimethylated H3 'Lys-36' (H3K36Me2), however, the relevance of this result remains unsure in vivo. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: has weak activity toward H3K9Me2 in absence of H3K4me3, while it has high activity toward H3K9me2 when binding H3K4me3.
