产品名称
METL8 Rabbit Polyclonal Antibody
别名
Methyltransferase-like protein 8 (EC 2.1.1.-)
蛋白名称
Methyltransferase-like protein 8 (EC 2.1.1.-)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q9H825
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9H825/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=228019
Mouse Swissprot No.
A2AUU0
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/A2AUU0/entry
特异性
This antibody detects endogenous levels of METL8 at Human, Mouse
稀释度
WB 1:500-2000 IHC 1:50-200
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
细胞定位
Mitochondrion . Mitochondrial protein: the cytoplasmic or nuclear localization observed by some groups is either the result of an incorrect localization caused by N-terminal tagging that interferes with mitochondrial targeting, or splice isoforms that lack the N-terminal mitochondrial transit sequence. .
功能
Mitochondrial S-adenosyl-L-methionine-dependent methyltransferase that mediates N(3)-methylcytidine modification of residue 32 of the tRNA anticodon loop of mitochondrial tRNA(Ser)(UCN) and tRNA(Thr) . N(3)-methylcytidine methylation modification regulates mitochondrial translation efficiency and is required for activity of the respiratory chain . N(3)-methylcytidine methylation of mitochondrial tRNA(Ser)(UCN) requires the formation of N(6)-dimethylallyladenosine(37) (i6A37) by TRIT1 as prerequisite . May also mediate N(3)-methylcytidine modification of mRNAs . The existence of N(3)-methylcytidine modification on mRNAs is however unclear, and additional evidences are required to confirm the role of the N(3)-methylcytidine-specific mRNA methyltransferase activity of METTL8 in vivo .
