产品名称
PIEZ1 Rabbit Polyclonal Antibody
别名
Piezo-type mechanosensitive ion channel component 1 (Membrane protein induced by beta-amyloid treatment) (Mib) (Protein FAM38A)
基因名称
PIEZO1 FAM38A KIAA0233
蛋白名称
Piezo-type mechanosensitive ion channel component 1 (Membrane protein induced by beta-amyloid treatment) (Mib) (Protein FAM38A)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=9780
Human Swissprot No.
Q92508
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q92508/entry
Mouse Swissprot No.
E2JF22
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/E2JF22/entry
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=361430
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q0KL00/entry
特异性
This antibody detects endogenous levels of PIEZ1 at Human, Mouse,Rat
稀释度
WB 1:500-2000 IHC 1:50-200
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
Expressed in numerous tissues. In normal brain, expressed exclusively in neurons, not in astrocytes. In Alzheimer disease brains, expressed in about half of the activated astrocytes located around classical senile plaques. In Parkinson disease substantia nigra, not detected in melanin-containing neurons nor in activated astrocytes. Expressed in erythrocytes (at protein level). Expressed in myoblasts (at protein level).
细胞定位
Endoplasmic reticulum membrane ; Multi-pass membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane . Cell membrane ; Multi-pass membrane protein . Cell projection, lamellipodium membrane . Cell membrane ; Multi-pass membrane protein. In erythrocytes, located in the plasma membrane (PubMed:22529292, PubMed:23479567). Accumulates at the leading apical lamellipodia of endothelial cells in response to shear stress (PubMed:25119035). Colocalizes with F-actin and MYH9 at the actomyosin cortex in myoblasts. .
功能
Pore-forming subunit of a mechanosensitive non-specific cation channel . Generates currents characterized by a linear current-voltage relationship that are sensitive to ruthenium red and gadolinium. Plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER, most probably in its activated state, and subsequent stimulation of calpain signaling . In the kidney, may contribute to the detection of intraluminal pressure changes and to urine flow sensing. Acts as shear-stress sensor that promotes endothelial cell organization and alignment in the direction of blood flow through calpain activation . Plays a key role in blood vessel formation and vascular structure in both development and adult physiology (By similarity). Acts as sensor of phosphatidylserine (PS) flipping at the plasma membrane and governs morphogenesis of muscle cells. In myoblasts, flippase-mediated PS enrichment at the inner leaflet of plasma membrane triggers channel activation and Ca2+ influx followed by Rho GTPases signal transduction, leading to assembly of cortical actomyosin fibers and myotube formation.
