产品名称
ZO3 Rabbit Polyclonal Antibody
蛋白名称
Tight junction protein ZO-3 (Tight junction protein 3) (Zona occludens protein 3) (Zonula occludens protein 3)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
O95049
Human Swissprot Link
https://www.uniprot.org/uniprotkb/O95049/entry
Mouse Swissprot No.
Q9QXY1
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9QXY1
免疫原
Synthesized peptide derived from part region of human protein
特异性
ZO3 Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is a member of the membrane-associated guanylate kinase-like (MAGUK) protein family which is characterized by members having multiple PDZ domains, a single SH3 domain, and a single guanylate kinase-like (GUK)-domain. In addition, members of the zonula occludens protein subfamily have an acidic domain, a basic arginine-rich region, and a proline-rich domain. The protein encoded by this gene plays a role in the linkage between the actin cytoskeleton and tight-junctions and also sequesters cyclin D1 at tight junctions during mitosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. This gene has a partial pseudogene on chromosome 1. [provided by RefSeq, May 2012],
组织表达
PCR rescued clones,Whole embryo,
细胞定位
Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, tight junction . Nucleus . Exhibits predominant nuclear expression in proliferating cells but is exclusively junctionally expressed after confluence is reached (PubMed:23608536). Shows an epithelial-specific tight junction localization in a TJP1/TJP2-dependent fashion (By similarity). .
功能
similarity:Belongs to the MAGUK family.,similarity:Contains 1 guanylate kinase-like domain.,similarity:Contains 1 PDZ (DHR) domain.,similarity:Contains 1 SH3 domain.,similarity:Contains 3 PDZ (DHR) domains.,subunit:Interacts with occludin, claudins and ZO-1. Interacts with INADL (By similarity). Interacts with UBN1.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
