产品名称
PRC1 (Phospho Thr470) Rabbit Polyclonal Antibody
别名
Protein regulator of cytokinesis 1
蛋白名称
Protein regulator of cytokinesis 1
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=9055
Human Swissprot No.
O43663
Human Swissprot Link
https://www.uniprot.org/uniprotkb/O43663/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=233406
Mouse Swissprot No.
Q99K43
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q99K43/entry
特异性
This antibody detects endogenous levels of PRC1 (Phospho Thr470) Rabbit pAb at Human, Mouse
稀释度
WB 1:500-2000 IHC 1:50-200
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
Overexpressed in bladder cancer cells (PubMed:17409436).
细胞定位
Nucleus . Cytoplasm. Cytoplasm, cytoskeleton, spindle pole . Midbody . Chromosome . Colocalized with KIF20B in the nucleus of bladder carcinoma cells at the interphase. Colocalized with KIF20B in bladder carcinoma cells at prophase, metaphase, early anaphase, at the midzone in late anaphase and at the contractile ring in telophase (PubMed:17409436). Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis. Co-localizes with PRC1 in early mitosis and at the spindle midzone from anaphase B to telophase (PubMed:15297875, PubMed:15625105). .
功能
function:KIF4A translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Acts as a microtubule-binding and bundling protein both in vivo and vitro. May function as an in vivo cyclin-CDK substrate.,PTM:Phosphorylated; very weak in G1/S phase cells. Much higher levels of phosphorylation are detected at later cell cycle phases, reaching a maximum during mitosis.,similarity:Belongs to the MAP65/ASE1 family.,subcellular location:Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis.,subunit:Interacts with the C-terminal Rho-GAP domain and the basic region of RACGAP1. The interaction with RACGAP1 inhibits its GAP activity towards Cdc42 in vitro, which may be required for maintaining normal spindle morphology. Interacts separately via its N-terminal region with the C-terminus of CENPE, KIF4A and KIF23 during late mitosis. Interacts with KIF14 and KIF20A.,
