产品名称
ALOX5 (Phospho Ser663) Rabbit Polyclonal Antibody
别名
Arachidonate 5-lipoxygenase (5-LO) (5-lipoxygenase) (EC 1.13.11.34)
蛋白名称
Arachidonate 5-lipoxygenase (5-LO) (5-lipoxygenase) (EC 1.13.11.34)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=240
Human Swissprot No.
P09917
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P09917/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=11689
Mouse Swissprot No.
P48999
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/P48999/entry
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25290
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/P12527/entry
特异性
This antibody detects endogenous levels of ALOX5 (Phospho Ser663) Rabbit pAb at Human, Mouse,Rat
稀释度
WB 1:500-2000 IHC 1:50-200
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
细胞定位
Cytoplasm . Nucleus matrix . Nucleus membrane ; Peripheral membrane protein . Cytoplasm, perinuclear region . Cytoplasm, cytosol . Nucleus envelope . Nucleus intermembrane space . Shuttles between cytoplasm and nucleus (PubMed:19233132). Found exclusively in the nucleus, when phosphorylated on Ser-272 (PubMed:18978352). Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association (PubMed:19233132, PubMed:3118366, PubMed:8245774, PubMed:16275640). .
功能
catalytic activity:Arachidonate + O(2) = leukotriene A(4) + H(2)O.,cofactor:Binds 1 iron ion per subunit.,cofactor:Binds 2 calcium ions per subunit.,function:Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.,pathway:Lipid metabolism; leukotriene A4 biosynthesis.,PTM:Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus.,similarity:Belongs to the lipoxygenase family.,similarity:Contains 1 lipoxygenase domain.,similarity:Contains 1 PLAT domain.,subcellular location:Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.,subunit:Interacts with ALOX5AP and LTC4S.,
