产品名称
Leptin Receptor (Phospho Tyr986) Rabbit Polyclonal Antibody
别名
Leptin receptor (LEP-R) (HuB219) (OB receptor) (OB-R) (CD antigen CD295)
蛋白名称
Leptin receptor (LEP-R) (HuB219) (OB receptor) (OB-R) (CD antigen CD295)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3953
Human Swissprot No.
P48357
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P48357/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=16847
Mouse Swissprot No.
P48356
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/P48356/entry
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=24536
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q62959/entry
特异性
This antibody detects endogenous levels of Leptin Receptor (Phospho Tyr986) Rabbit pAb at Human, Mouse,Rat
稀释度
WB 1:500-2000 IHC 1:50-200
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus, but also in skeletal muscle. Detected in fundic and antral epithelial cells of the gastric mucosa (PubMed:19159218). Isoform B and isoform A are expressed by NK cells (at protein level) (PubMed:12504075).
细胞定位
Cell membrane ; Single-pass type I membrane protein . Basolateral cell membrane .; [Isoform E]: Secreted .
功能
domain:The box 1 motif is required for JAK interaction and/or activation.,domain:The cytoplasmic domain may be essential for intracellular signal transduction by activation of JAK tyrosine kinase and STATs.,domain:The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.,function:Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway.,PTM:On ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation and, for interaction with SOCS3 (By similarity). Phosphorylation on Tyr-1141 is required for STAT3 binding/activation.,similarity:Belongs to the type I cytokine receptor family. Type 2 subfamily.,similarity:Contains 1 Ig-like (immunoglobulin-like) domain.,similarity:Contains 4 fibronectin type-III domains.,subunit:On leptin stimulation, homodimerizes. The phosphorylated receptor binds a number of SH2 domain-containing proteins such as STAT3, PTPN11, and SOCS3 (By similarity). Interaction with SOCS3 inhibits LRb signaling.,tissue specificity:Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus.,
