产品名称
CARD15/NOD2 Rabbit Polyclonal Antibody
蛋白名称
Nucleotide-binding oligomerization domain-containing protein 2 (Caspase recruitment domain-containing protein 15) (Inflammatory bowel disease protein 1)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=64127
Human Swissprot No.
Q9HC29
Human Swissprot Link
https://www.uniprot.org/uniprot/Q9HC29
Mouse Swissprot No.
Q8K3Z0
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q8K3Z0/entry
特异性
This antibody detects endogenous levels of CARD15/NOD2 at Human
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
Mainly expressed in monocytes (PubMed:11087742). Expressed also in intestinal mucosa, mainly in Paneth cells and, at lower extent, in the glandular epithelium (PubMed:22700971).
细胞定位
Cell membrane . Cytoplasm . Membrane . Mitochondrion . Basolateral cell membrane .
功能
Pattern recognition receptor (PRR) that detects bacterial peptidoglycan fragments and other danger signals and plays an important role in gastrointestinal immunity . Upon stimulation by muramyl dipeptide (MDP), a fragment of bacterial peptidoglycan, binds the proximal adapter receptor-interacting RIPK2, which recruits ubiquitin ligases as XIAP, BIRC2, BIRC3, INAVA and the LUBAC complex, triggering activation of MAP kinases and activation of NF-kappa-B signaling . This in turn leads to the transcriptional activation of hundreds of genes involved in immune response. Required for MDP-induced NLRP1-dependent CASP1 activation and IL1B release in macrophages . Component of an autophagy-mediated antibacterial pathway together with ATG16L1 . Plays also a role in sensing single-stranded RNA (ssRNA) from viruses. Interacts with mitochondrial antiviral signaling/MAVS, leading to activation of interferon regulatory factor-3/IRF3 and expression of type I interferon . Besides recognizing pathogens, participates in surveillance of cellular homeostasis through sensing and binding to the cytosolic metabolite sphingosine-1-phosphate and then initating inflammation process .
