产品名称
ZO3 Rabbit Polyclonal Antibody
蛋白名称
Tight junction protein ZO-3 (Tight junction protein 3) (Zona occludens protein 3) (Zonula occludens protein 3)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
O95049
Human Swissprot Link
https://www.uniprot.org/uniprotkb/O95049/entry
Mouse Swissprot No.
Q9QXY1
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9QXY1
免疫原
Synthesized peptide derived from part region of human protein
特异性
ZO3 Polyclonal Antibody detects endogenous levels of protein.
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is a member of the membrane-associated guanylate kinase-like (MAGUK) protein family which is characterized by members having multiple PDZ domains, a single SH3 domain, and a single guanylate kinase-like (GUK)-domain. In addition, members of the zonula occludens protein subfamily have an acidic domain, a basic arginine-rich region, and a proline-rich domain. The protein encoded by this gene plays a role in the linkage between the actin cytoskeleton and tight-junctions and also sequesters cyclin D1 at tight junctions during mitosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. This gene has a partial pseudogene on chromosome 1. [provided by RefSeq, May 2012],
组织表达
PCR rescued clones,Whole embryo,
细胞定位
Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, tight junction . Nucleus . Exhibits predominant nuclear expression in proliferating cells but is exclusively junctionally expressed after confluence is reached (PubMed:23608536). Shows an epithelial-specific tight junction localization in a TJP1/TJP2-dependent fashion (By similarity). .
功能
similarity:Belongs to the MAGUK family.,similarity:Contains 1 guanylate kinase-like domain.,similarity:Contains 1 PDZ (DHR) domain.,similarity:Contains 1 SH3 domain.,similarity:Contains 3 PDZ (DHR) domains.,subunit:Interacts with occludin, claudins and ZO-1. Interacts with INADL (By similarity). Interacts with UBN1.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
