产品名称
HELB Rabbit Polyclonal Antibody
蛋白名称
DNA helicase B (hDHB) (EC 3.6.4.12)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=92797
Human Swissprot No.
Q8NG08
Human Swissprot Link
https://www.uniprot.org/uniprot/Q8NG08
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=117599
Mouse Swissprot No.
Q6NVF4
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q6NVF4/entry
特异性
This antibody detects endogenous levels of HELB at Human, Mouse
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
Highly expressed in testis and thymus and weakly in liver, spleen, kidney and brain.
细胞定位
Nucleus . Cytoplasm . Chromosome . Predominantly nuclear (PubMed:15146062). Phosphorylation at Ser-967 by CDK2 during the G1/S transition results in its nuclear export into the cytoplasm as cells approach and progress through S phase (PubMed:15146062). Following DNA damage, recruited to sites of double-strand breaks by the RPA complex (PubMed:26774285). Recruited to chromatin following DNA damage induced by UV irradiation, or camptothecin or hydroxyurea treatment (PubMed:22194613). .
功能
5'-3' DNA helicase involved in DNA damage response by acting as an inhibitor of DNA end resection . Recruitment to single-stranded DNA (ssDNA) following DNA damage leads to inhibit the nucleases catalyzing resection, such as EXO1, BLM and DNA2, possibly via the 5'-3' ssDNA translocase activity of HELB . As cells approach S phase, DNA end resection is promoted by the nuclear export of HELB following phosphorylation . Acts independently of TP53BP1 . Unwinds duplex DNA with 5'-3' polarity. Has single-strand DNA-dependent ATPase and DNA helicase activities. Prefers ATP and dATP as substrates . During S phase, may facilitate cellular recovery from replication stress .
