产品名称
SPAG5 Rabbit Polyclonal Antibody
蛋白名称
Sperm-associated antigen 5 (Astrin) (Deepest) (Mitotic spindle-associated protein p126) (MAP126)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q96R06
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q96R06/entry
Mouse Swissprot No.
Q7TME2
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q7TME2
免疫原
Synthesized peptide derived from part region of human protein
特异性
SPAG5 Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a protein associated with the mitotic spindle apparatus. The encoded protein may be involved in the functional and dynamic regulation of mitotic spindles. [provided by RefSeq, Jul 2008],
组织表达
Highly expressed in testis. Detected at low levels in placenta, liver, pancreas, thymus and colon.
细胞定位
Cytoplasm . Cytoplasm, cytoskeleton . Cytoplasm, cytoskeleton, spindle . Cytoplasm, cytoskeleton, spindle pole . Chromosome, centromere, kinetochore . Midbody. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic granule. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite . Colocalizes with PCM1 at centriolar satellites throughout the cell cycle (PubMed:26297806). In a punctate pattern in interphase cells. During mitosis, detected at spindle poles during prophase, throughout the spindle in metaphase and anaphase, and at midzone microtubules in anaphase and telophase (PubMed:27462074). Efficient targeting to the mitotic spindle may depend upon phosphorylation by GSK3B. Detected on kinetochores of chromosomes that have congressed. The astrin (SPAG5)-kinastrin (SKAP) complex localizes to the microtubule plus ends (By similarity). In non-mitotic non-stressed cells, shows a microtubuli pattern. In arsenite-stressed cells, accumulates in stress granules. .
功能
function:Necessary for normal spindle formation during mitosis.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,subcellular location:In a punctate pattern in interphase cells. During mitosis, detected at spindle poles during prophase, throughout the spindle in metaphase and anaphase, and at midzone microtubules in anaphase and telophase. Detected on kinetochores of chromosomes that have congressed.,subunit:Homodimer, with a globular head domain and a long stalk. Homo-oligomer; the globular head domains associate, resulting in aster-like structures. Binds to microtubules in the mitotic spindle.,tissue specificity:Highly expressed in testis. Detected at low levels in placenta, liver, pancreas, thymus and colon.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
