产品名称
QTRT1 Rabbit Polyclonal Antibody
蛋白名称
Queuine tRNA-ribosyltransferase (EC 2.4.2.29) (Guanine insertion enzyme) (tRNA-guanine transglycosylase)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=81890
Human Swissprot No.
Q9BXR0
Human Swissprot Link
https://www.uniprot.org/uniprot/Q9BXR0
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=60507
Mouse Swissprot No.
Q9JMA2
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q9JMA2/entry
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=64016
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q4QR99/entry
特异性
This antibody detects endogenous levels of QTRT1 at Human, Mouse,Rat
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
细胞定位
Cytoplasm . Mitochondrion outer membrane ; Peripheral membrane protein ; Cytoplasmic side . Weakly associates with mitochondria, possibly via QTRT2. .
功能
Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) . Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product (By similarity).
