产品名称
SH3B4 Rabbit Polyclonal Antibody
基因名称
SH3BP4 BOG25 EHB10 TTP
蛋白名称
SH3 domain-binding protein 4 (EH-binding protein 10) (Transferrin receptor-trafficking protein)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q9P0V3
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9P0V3/entry
Mouse Swissprot No.
Q921I6
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q921I6
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941Q9JJS5
免疫原
Synthesized peptide derived from part region of human protein around amino acid site 145
特异性
SH3B4 Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a protein with 3 Asn-Pro-Phe (NPF) motifs, an SH3 domain, a PXXP motif, a bipartite nuclear targeting signal, and a tyrosine phosphorylation site. This protein is involved in cargo-specific control of clathrin-mediated endocytosis, specifically controlling the internalization of a specific protein receptor. [provided by RefSeq, Jul 2008],
组织表达
Expressed in all tissues tested with higher expression in pancreas. Expressed by retinal pigment epithelial cells (at protein level).
细胞定位
Membrane, clathrin-coated pit. Cytoplasmic vesicle, clathrin-coated vesicle. Nucleus . Specifically associated with transferrin receptor-containing clathrin-coated pits and clathrin-coated vesicles. May also localize to the nucleus.
功能
domain:The SH3 domain mediates localization to the clathrin-coated pits and vesicles. The SH3 domain mediates interaction with DNM2 and the cytoplasmic part of TFRC with a lower affinity.,function:Functions in transferrin receptor internalization at the plasma membrane through a cargo-specific control of clathrin-mediated endocytosis.,miscellaneous:Overexpression or depletion of SH3BP4 result in a specific decrease of the transferrin receptor endocytosis that can be rescued by DNM2 overexpression.,PTM:Phosphorylated upon EGF stimulation. Phosphorylation prevents interaction with DNM2.,similarity:Contains 1 SH3 domain.,subcellular location:Specifically associated with transferrin receptor-containing clathrin-coated pits and clathrin-coated vesicles. May also localize to the nucleus.,subunit:Homodimer or homooligomer. Interacts with DNM2, EPS15, clathrin, the adapter protein complex 2/AP-2 and TFRC.,tissue specificity:Expressed in all tissues tested with higher expression in pancreas. Expressed by retinal pigment epithelial cells (at protein level).,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
